ECL Substrate

LightWave™ is our product line of ECL HRP substrates for Western blotting.

Our double enhancer proprietary technology allows for modulation of signal intensity and signal duration.

Each LightWave™ substrate is at the top of its respective market segment regarding performance/price ratio.

Product Details

Properties All LightWave substrates are:
Stable for 1 year at RT
Low picogram detection
Ideal for routinary analysis
Working solution stable for at least three days
The best entry level ECL substrate on the market
Signal duration 5 hours
LightWave™ has:
Signal intensity: medium
Signal duration: medium
Protein abundance: high
Storage/expiry: one year at room temperature (max. 25°C)
Characteristics Sample two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
Primary antibody rabbit-anti Human HDAC-1 (abcam®) 1:2000
Secondary antibody goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:20000
Exposure time 180 seconds
Compatible with all chemiluminescence imagers and X-ray film detection
Optimized for attaining low background and high signal to noise ratio
Applications
Western Blotting
Size/ SKU/ Item Code LW0001 LightWave Western Blotting Substrate 10ml ECL Substrate

LW0002 LightWave Western Blotting Substrate 250ml ECL Substrate

LW0003 LightWave Plus Western Blotting Substrate 10ml ECL Substrate

LW0004 LightWave Plus Western Blotting Substrate 250ml ECL Substrate

LW0005 LightWave Max Western Blotting High Sensitive Substrate 10ml ECL Substrate

LW0006 LightWave Max Western Blotting High Sensitive Substrate 100ml ECL Substrate

 

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